Buffered Peptone Water

Buffered peptone water is used in standard methods.

Buffered peptone water. The phosphate salts provide buffering capacity to maintain the ph. Iso 6579 iso 21528 iso 22964 fda bam and ep 86 g irradiated readybag find msds or sds a coa data sheets and more information. Peptone in the media supplies nitrogenous compounds needed for the growth of bacteria. Buffered peptone water bpw iso is not intended for use in the diagnosis of disease or other conditions in humans.

Cells may be present in a test food sample. Pre enrichment with buffered peptone water maintained a high ph over a period of 24 hours incubation. Rich in nutrients the medium. This pre enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation.

Vegetable tissue has a low buffering capacity and the medium overcame this problem. Buffered peptone water acc. Formulated to iso 6579 1 2017 iso 22694 2017 iso 21528 1 2017 and iso 12950 2010. Pre enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity.

Supports high growth and recovery rate. This pre enrichment medium is designed to help sublethally damaged salmonellae recover before introducing them into a selective medium. Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Buffered peptone water is a pre enrichment medium designed to help recovery of sub lethally damaged salmonellae before transfer to a selective medium.

A shortened enrichment time of 6 hours was investigated 5 but in circumstances where heavily contaminated materials were examined the addition of 0 1g of malachite green per litre of buffered peptone water. Low numbers and or sub lethally injured salmonella spp. Buffered peptone water is used for the detection of microorganisms and it improves the detection of bacteria from raw ingredients finished product and samples taken from the food processing environment this solution is an ideal medium in which to retain microbial viability and promote growth particularly with samples taken from surfaces and carcasses such as those in poultry processing plants. Buffered peptone water maintains a high ph over the pre enrichment period and allows in repair of injured cells that may be sensitive to low ph.

This is particularly important for vegetable specimens which have a low buffering capacity. Buffered peptone water is a non selective preenrichment broth that promotes the recovery of those bacteria that may be injured.